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qPCR-primarily based quantitative microbiome profiling is applicable also for samples containing a substantial quantity of host or non-bacterial DNA, where bacterial density can't be reliably believed by whole DNA generate [5].

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Importantly, qPCR-centered quantitative microbiome profiling enjoys the following conceptual and realistic Advantages in excess of other techniques:

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Using relative abundance from future era sequencing (NGS) facts may lead to misinterpretations of microbial Neighborhood buildings as on account of compositionality, the relative abundances on the taxa being mutually dependent. Which means that a boost of 1 taxon inevitably causes the concurrent lower of one other(s). Since the variations of factors are mutually dependent, large Wrong discovery fees occur when compositional facts are analyzed making use of standard statistical strategies [1].

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